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  • KD/KO Validated

AURKA Monoclonal antibody

AURKA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF/ICC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

CloneNo.

1F4B10

Cat no : 66757-1-Ig

Synonyms

AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7


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Tested Applications

Positive WB detected inhuman testis tissue, HeLa cells, NCCIT cells, rat testis tissue, mouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66757-1-Ig targets AURKA in WB, IHC, IF/ICC, CoIP, ChIP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen AURKA fusion protein Ag20096 相同性解析による交差性が予測される生物種
Full Name aurora kinase A
Calculated molecular weight 46 kDa
Observed molecular weight 46-52 kDa
GenBank accession numberBC002499
Gene symbol AURKA
Gene ID (NCBI) 6790
RRIDAB_2882103
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

Protocols

Product Specific Protocols
WB protocol for AURKA antibody 66757-1-IgDownload protocol
IHC protocol for AURKA antibody 66757-1-IgDownload protocol
IF protocol for AURKA antibody 66757-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

PUF60/AURKA Axis Contributes to Tumor Progression and Malignant Phenotypes in Bladder Cancer.

Authors - Qian Long
humanIHC

Front Oncol

Integrated Analysis of Transcriptome Data Revealed AURKA and KIF20A as Critical Genes in Medulloblastoma Progression.

Authors - Bo Liang
humanWB

Front Oncol

Proteomics and phosphoproteomics of chordoma biopsies reveal alterations in multiple pathways and aberrant kinases activities

Authors - Jing Hang
  • KD Validated
humanWB

Biochem Pharmacol

Palmatine induces G2/M phase arrest and mitochondrial-associated pathway apoptosis in colon cancer cells by targeting AURKA.

Authors - Xiaojiang Liu
humanWB

Carcinogenesis

ARID3A promotes the development of colorectal cancer by upregulating AURKA.

Authors - Jing Tang
humanWB

Biochim Biophys Acta Mol Basis Dis

Blocking AURKA with MK-5108 attenuates renal fibrosis in chronic kidney disease.

Authors - Mingzhu Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sarah-Eve (Verified Customer) (06-02-2023)

Works well, WB dilution 1:2000

  • Applications: Western Blot
  • Cell Tissue Type: Human pulmonary artery smooth muscle cells