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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using AURKA Monoclonal antibody (66757-1-Ig)

    WB analysis of human testis using 66757-1-Ig (same clone as 66757-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AURKA Monoclonal antibody (66757-1-Ig)

    WB analysis using 66757-1-Ig (same clone as 66757-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using AURKA Monoclonal antibody (66757-1-Ig)

    WB analysis of rat testis using 66757-1-Ig (same clone as 66757-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using AURKA Monoclonal antibody (66757-1-Ig)

    WB analysis of mouse testis using 66757-1-Ig (same clone as 66757-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using AURKA Monoclonal antibody (66757-1-Ig)

    WB analysis of HeLa using 66757-1-Ig (same clone as 66757-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using AURKA Monoclonal antibody (66757-1-Ig)

    WB analysis of NCCIT using 66757-1-Ig (same clone as 66757-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AURKA Monoclonal antibody (66757-1-Ig)

    IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AURKA Monoclonal antibody (66757-1-Ig)

    IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AURKA Monoclonal antibody (66757-1-Ig)

    IF Staining of HeLa using 66757-1-Ig (same clone as 66757-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AURKA antibody (66757-1-Ig, Clone: 1F4B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50265-1

    Cytometric bead array standard curve of MP50265-1

    Cytometric bead array standard curve of MP50265-1, AURKA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66757-2-PBS. Detection antibody: 66757-1-PBS. Standard:Ag20096. Range: 0.391-50 ng/mL.

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  • Featured Product
  • KD/KO Validated

AURKA Monoclonal antibody, PBS Only (Detector)

AURKA Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66757-1-PBS
Clone No.1F4B10

Host / Isotype

Mouse / IgG3

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

Aurora A, AURK, AURA, ARK-1, AIRK1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66757-1-PBS targets AURKA as part of a matched antibody pair:

MP50265-1: 66757-2-PBS capture and 66757-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen AURKA fusion protein Ag20096 相同性解析による交差性が予測される生物種
Full Name aurora kinase A
Calculated molecular weight 46 kDa
Observed molecular weight 46-52 kDa
GenBank accession numberBC002499
Gene Symbol AURKA
Gene ID (NCBI) 6790
RRIDAB_2882103
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14965
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).