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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AURKA Recombinant antibody (82906-1-RR)

    WB analysis using 82906-1-RR (same clone as 82906-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82906-1-RR (AURKA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82906-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AURKA Recombinant antibody (82906-1-RR)

    IF Staining of HeLa using 82906-1-RR (same clone as 82906-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AURKA antibody (82906-1-RR, Clone: 230087H3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82906-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AURKA Recombinant antibody (82906-1-RR)

    IF Staining of HeLa using 82906-1-RR (same clone as 82906-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AURKA antibody (82906-1-RR, Clone: 230087H3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82906-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82906-1-RR

    Affinity and Kinetic Characterization of 82906-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82906-1-RR against Human AURKA were performed. The affinity constant is below 1 pM.

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AURKA Recombinant antibody, PBS Only

AURKA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82906-1-PBS
Clone No.230087H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Aurora A, AURK, AURA, ARK-1, AIRK1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82906-1-PBS targets AURKA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AURKA fusion protein Ag0362 相同性解析による交差性が予測される生物種
Full Name aurora kinase A
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC002499
Gene Symbol AURKA
Gene ID (NCBI) 6790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14965
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).