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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AURKA Recombinant antibody

AURKA Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230087H3

Cat no : 82906-1-RR

Synonyms

Aurora A, AURK, AURA, ARK-1, AIRK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AURKA Recombinant antibody (82906-1-RR)

    WB analysis using 82906-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82906-1-RR (AURKA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AURKA Recombinant antibody (82906-1-RR)

    IF Staining of HeLa using 82906-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AURKA antibody (82906-1-RR, Clone: 230087H3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AURKA Recombinant antibody (82906-1-RR)

    IF Staining of HeLa using 82906-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AURKA antibody (82906-1-RR, Clone: 230087H3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Affinity and Kinetic Characterization of 82906-1-RR

    Affinity and Kinetic Characterization of 82906-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82906-1-RR against Human AURKA were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells, HeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82906-1-RR targets AURKA in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AURKA fusion protein Ag0362 相同性解析による交差性が予測される生物種
Full Name aurora kinase A
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC002499
Gene symbol AURKA
Gene ID (NCBI) 6790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

Protocols

Product Specific Protocols
WB protocol for AURKA antibody 82906-1-RRDownload protocol
IF protocol for AURKA antibody 82906-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols