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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Acetyl-Histone H2A (Lys9) Recombinant antibody (82823-2-RR)

    WB analysis of HeLa using 82823-2-RR

    Trichostain A treated and untreated HeLa cell lysates were subjected to SDS PAGE followed by western blot with 82823-2-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Dot Blot experiment of peptide using Acetyl-Histone H2A (Lys9) Recombinant antibody (82823-2-RR)

    Dot Blot experiment of peptide using 82823-2-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2A (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H2AK9ac. Column 2: Unmodified H2AK9. Column 3: H2AK5ac. Column 4: Unmodified H2AK5. Column 5: H2AK13Ac. Column 6: Unmodified H2AK13. Column 7: H2AK15ac. Column 8:Unmodified H2AK15. Column 9: Blank(PBS).

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Acetyl-Histone H2A (Lys9) Recombinant antibody

Acetyl-Histone H2A (Lys9) Recombinant Antibody for WB, Dot Blot, ELISA
Cat No. 82823-2-RR
Clone No.1O17

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Dot Blot, ELISA

Synonyms

HIST1H2AA, H2AK9Ac, H2AFR, H2A-clustered histone 1, H2AC1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82823-2-RR targets Acetyl-Histone H2A (Lys9) in WB, Dot Blot, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H2aa
Observed molecular weight16 kDa
GenBank accession numberBC062211
Gene Symbol HIST1H2AA
Gene ID (NCBI) 221613
RRIDAB_3670564
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96QV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Acetyl-Histone H2A (Lys9) antibody 82823-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols