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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Acetyl-Histone H2A (Lys9) Recombinant antibody (82823-2-RR)

    WB analysis of HeLa using 82823-2-RR (same clone as 82823-2-PBS)

    Trichostain A treated and untreated HeLa cell lysates were subjected to SDS PAGE followed by western blot with 82823-2-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82823-2-PBS in a different storage buffer formulation.

  • Dot Blot experiment of peptide using Acetyl-Histone H2A (Lys9) Recombinant antibody (82823-2-RR)

    Dot Blot experiment of peptide using 82823-2-RR (same clone as 82823-2-PBS)

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2A (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H2AK9ac. Column 2: Unmodified H2AK9. Column 3: H2AK5ac. Column 4: Unmodified H2AK5. Column 5: H2AK13Ac. Column 6: Unmodified H2AK13. Column 7: H2AK15ac. Column 8:Unmodified H2AK15. Column 9: Blank(PBS). This data was developed using the same antibody clone with 82823-2-PBS in a different storage buffer formulation.

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Acetyl-Histone H2A (Lys9) Recombinant antibody, PBS Only

Acetyl-Histone H2A (Lys9) Uni-rAbTM Recombinant Antibody for WB, Dot Blot, Indirect ELISA
Cat No. 82823-2-PBS
Clone No.1O17

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Dot Blot, Indirect ELISA

Synonyms

HIST1H2AA, H2AK9Ac, H2AFR, H2A-clustered histone 1, H2AC1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82823-2-PBS targets Acetyl-Histone H2A (Lys9) in WB, Dot Blot, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H2aa
Observed molecular weight16 kDa
GenBank accession numberBC062211
Gene Symbol HIST1H2AA
Gene ID (NCBI) 221613
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96QV6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.