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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Acetyl-Histone H2B (Lys5) Recombinant antibody

Acetyl-Histone H2B (Lys5) Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230539G10

Cat no : 83171-4-RR

Synonyms

HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Acetyl-Histone H2B (Lys5) Recombinant antibody (83171-4-RR)

    WB analysis of NIH/3T3 using 83171-4-RR

    Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83171-4-RR (Acetyl-Histone H2B (Lys5) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2B (Lys5) Recombinant antibody (83171-4-RR)

    IF Staining of HeLa using 83171-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys5) antibody (83171-4-RR, Clone: 230539G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2B (Lys5) Recombinant antibody (83171-4-RR)

    IF Staining of HeLa using 83171-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys5) antibody (83171-4-RR, Clone: 230539G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive IF/ICC detected inHeLa cells, Trichostatin A treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83171-4-RR targets Acetyl-Histone H2B (Lys5) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 2, H2be
Calculated molecular weight 14 kDa
Observed molecular weight15 kDa
GenBank accession numberBC005827
Gene symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Acetyl-Histone H2B (Lys5) antibody 83171-4-RRDownload protocol
IF protocol for Acetyl-Histone H2B (Lys5) antibody 83171-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols