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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Acetyl-Histone H2B (Lys5) Recombinant antibody

Acetyl-Histone H2B (Lys5) Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230539G10

Cat no : CL488-83171-4

Synonyms

HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Hela cells using CoraLite® Plus 488-conjugated Acetyl-Histone H2B ( (CL488-83171-4)

    IF Staining of Hela using CL488-83171-4

    Immunofluorescent analysis of (4% PFA) fixed Hela cells using CoraLite® Plus 488 Acetyl-Histone H2B (Lys5) antibody (CL488-83171-4, Clone: 230539G10 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated Acetyl-Histone H2B ( (CL488-83171-4)

    FC experiment of HeLa using CL488-83171-4

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Acetyl-Histone H2b (Lys5) Recombinant Antibody (CL488-83171-4, Clone:230539G10)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

Positive IF/ICC detected inHela cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83171-4 targets Acetyl-Histone H2B (Lys5) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 2, H2be
Calculated molecular weight 14 kDa
Observed molecular weight15 kDa
GenBank accession numberBC005827
Gene symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Acetyl-Histone H2B (Lys5) antibody CL488-83171-4Download protocol
Standard Protocols
Click here to view our Standard Protocols