Acetyl-Histone H3 (Lys9) Recombinant antibody, PBS Only

HEK-293 cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

Trichostatin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (HIST1H3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84543-1-RR, Clone: 241596F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.