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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Acetyl-Histone H3 (Lys9) Recombinant antibody

Acetyl-Histone H3 (Lys9) Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

241596F9

Cat no : 84543-1-RR

Synonyms

HIST1H3A, 241596F9, Histone-H3, HistoneH3, Histone H3

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Acetyl-Histone H3 (Lys9) Recombinant antibody (84543-1-RR)

    WB analysis of HEK-293 using 84543-1-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Acetyl-Histone H3 (Lys9) Recombinant antibody (84543-1-RR)

    WB analysis of HeLa using 84543-1-RR

    Trichostatin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (HIST1H3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H3 (Lys9) Recombinant antibody (84543-1-RR)

    IF Staining of HeLa using 84543-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84543-1-RR, Clone: 241596F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Dot Blot experiment of peptide using Acetyl-Histone H3 (Lys9) Recombinant antibody (84543-1-RR)

    Dot Blot experiment of peptide using 84543-1-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

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Tested Applications

Positive WB detected inTrichostatin A treated HeLa cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84543-1-RR targets Acetyl-Histone H3 (Lys9) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight17 kDa
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Acetyl-Histone H3 (Lys9) antibody 84543-1-RRDownload protocol
IF protocol for Acetyl-Histone H3 (Lys9) antibody 84543-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols