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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Adiponectin receptor Recombinant antibody (85121-4-RR)

    WB analysis using 85121-4-RR (same clone as 85121-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85121-4-RR (ADIPOR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85121-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using Adiponectin receptor Recombinant antibody (85121-4-RR)

    IP experiment of mouse liver using 85121-4-RR (same clone as 85121-4-PBS)

    IP result of anti-Adiponectin receptor (IP:85121-4-RR, 4ug; Detection:85121-4-RR 1:2000) with mouse liver tissue lysate 2400 ug. This data was developed using the same antibody clone with 85121-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using Adiponectin receptor Recombinant antibody (85121-4-RR)

    IHC staining of rat liver using 85121-4-RR (same clone as 85121-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85121-4-RR (Adiponectin receptor antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85121-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Adiponectin receptor Recombinant antibody (85121-4-RR)

    IHC staining of mouse liver using 85121-4-RR (same clone as 85121-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85121-4-RR (Adiponectin receptor antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85121-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Adiponectin receptor Recombinant antibody (85121-4-RR)

    IHC staining of mouse liver using 85121-4-RR (same clone as 85121-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85121-4-RR (Adiponectin receptor antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85121-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using Adiponectin receptor Recombinant antibody (85121-4-RR)

    IHC staining of rat liver using 85121-4-RR (same clone as 85121-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85121-4-RR (Adiponectin receptor antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85121-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Adiponectin receptor Recombinant antibody (85121-4-RR)

    IF Staining of mouse liver using 85121-4-RR (same clone as 85121-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Adiponectin receptor antibody (85121-4-RR, Clone: 242777F3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85121-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Adiponectin receptor Recombinant antibody (85121-4-RR)

    IF Staining of mouse liver using 85121-4-RR (same clone as 85121-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Adiponectin receptor antibody (85121-4-RR, Clone: 242777F3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85121-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85121-4-RR

    Affinity and Kinetic Characterization of 85121-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85121-4-RR against Human Adiponectin receptor were performed. The affinity constant is 2.85 nM.

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Adiponectin receptor Recombinant antibody, PBS Only

Adiponectin receptor Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 85121-4-PBS
Clone No.242777F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

ADIPOR2, ADIPOR1, Adiponectin receptor protein 2, adiponectin receptor 2, ACDCR2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85121-4-PBS targets Adiponectin receptor in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Adiponectin receptor fusion protein Ag5744 相同性解析による交差性が予測される生物種
Full Name adiponectin receptor 2
Calculated molecular weight 44 kDa
Observed molecular weight45 kDa
GenBank accession numberBC051858
Gene Symbol Adiponectin receptor
Gene ID (NCBI) 79602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86V24
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin (ADIPOR1 and ADIPOR2) have been identified. They mediate increased AMPK, MAPK, and PPARA ligand activity in response to adiponectin. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. The human ADIPOR2 gene maps to chromosome 12p13.31, and encodes a 386-amino acid protein with a molecular weight of 44 kDa. AdipoR2 may also exist as stable ~ 60 kDa dimers (PMID: 18842004). This antibody raised against 1-147aa of human AdipoR2 may cross-react with AdipoR1.