Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, L02 cells, rat uterus tissue, human placenta tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | rat liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85121-4-RR targets Adiponectin receptor in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Adiponectin receptor fusion protein Ag5744 相同性解析による交差性が予測される生物種 |
| Full Name | adiponectin receptor 2 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC051858 |
| Gene Symbol | Adiponectin receptor |
| Gene ID (NCBI) | 79602 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q86V24 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin (ADIPOR1 and ADIPOR2) have been identified. They mediate increased AMPK, MAPK, and PPARA ligand activity in response to adiponectin. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. The human ADIPOR2 gene maps to chromosome 12p13.31, and encodes a 386-amino acid protein with a molecular weight of 44 kDa. AdipoR2 may also exist as stable ~ 60 kDa dimers (PMID: 18842004). This antibody raised against 1-147aa of human AdipoR2 may cross-react with AdipoR1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Adiponectin receptor antibody 85121-4-RR | Download protocol |
| IHC protocol for Adiponectin receptor antibody 85121-4-RR | Download protocol |
| IF protocol for Adiponectin receptor antibody 85121-4-RR | Download protocol |
| IP protocol for Adiponectin receptor antibody 85121-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |