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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Aldolase C Monoclonal antibody, PBS Only

Aldolase C Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5H7A5

Cat no : 66120-1-PBS

Synonyms

ALDOC, Aldolase C, Brain type aldolase, Fructose bisphosphate aldolase C

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Aldolase C Monoclonal antibody (66120-1-Ig)

    WB analysis using 66120-1-Ig (same clone as 66120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    WB analysis of human heart using 66120-1-Ig (same clone as 66120-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    WB analysis of human brain using 66120-1-Ig (same clone as 66120-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    IHC staining of human liver cancer using 66120-1-Ig (same clone as 66120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    IHC staining of human liver cancer using 66120-1-Ig (same clone as 66120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    IHC staining of mouse brain using 66120-1-Ig (same clone as 66120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    IHC staining of mouse liver using 66120-1-Ig (same clone as 66120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    IHC staining of mouse liver using 66120-1-Ig (same clone as 66120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    IHC staining of mouse brain using 66120-1-Ig (same clone as 66120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Aldolase C Monoclonal antibody (66120-1-Ig)

    IF Staining of HepG2 using 66120-1-Ig (same clone as 66120-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldolase C antibody (66120-1-Ig, Clone: 5H7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66120-1-PBS targets Aldolase C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Aldolase C fusion protein Ag6779 相同性解析による交差性が予測される生物種
Full Name aldolase C, fructose-bisphosphate
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC003613
Gene symbol Aldolase C
Gene ID (NCBI) 230
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).