Aldolase C Monoclonal antibody
Aldolase C Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
5H7A5
Cat no : 66120-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, human brain tissue, human heart tissue, HepG2 cells, K-562 cells, HSC-T6 cells, Pig brain tissue, Mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue |
Positive IHC detected in | human liver cancer tissue, mouse brain tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66120-1-Ig targets Aldolase C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Aldolase C fusion protein Ag6779 相同性解析による交差性が予測される生物種 |
Full Name | aldolase C, fructose-bisphosphate |
Calculated molecular weight | 39 kDa |
Observed molecular weight | 39 kDa |
GenBank accession number | BC003613 |
Gene symbol | Aldolase C |
Gene ID (NCBI) | 230 |
RRID | AB_2881519 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).
Protocols
Product Specific Protocols | |
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WB protocol for Aldolase C antibody 66120-1-Ig | Download protocol |
IHC protocol for Aldolase C antibody 66120-1-Ig | Download protocol |
IF protocol for Aldolase C antibody 66120-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |