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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Aldolase C Monoclonal antibody (66120-1-Ig)

    WB analysis using 66120-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of human heart tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    WB analysis of human heart using 66120-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    WB analysis of human brain using 66120-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    IHC staining of human liver cancer using 66120-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    IHC staining of human liver cancer using 66120-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    IHC staining of mouse liver using 66120-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    IHC staining of mouse liver using 66120-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    IHC staining of mouse brain using 66120-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Aldolase C Monoclonal antibody (66120-1-Ig)

    IHC staining of mouse brain using 66120-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Aldolase C Monoclonal antibody (66120-1-Ig)

    IF Staining of HepG2 using 66120-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldolase C antibody (66120-1-Ig, Clone: 5H7A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Aldolase C Monoclonal antibody (66120-1-Ig)

    IF Staining of HepG2 using 66120-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldolase C antibody (66120-1-Ig, Clone: 5H7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Aldolase C Monoclonal antibody

Aldolase C Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66120-1-Ig
Clone No.5H7A5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ALDOC, 5H7A5, ALDC, ALDO, Brain-type aldolase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, human brain tissue, human heart tissue, HepG2 cells, K-562 cells, HSC-T6 cells, Pig brain tissue, Mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IHC detected inhuman liver cancer tissue, mouse brain tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66120-1-Ig targets Aldolase C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6779

Product name: Recombinant human ALDOC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-364 aa of BC003613

Sequence: MPHSYPALSAEQKKELSDIALRIVAPGKGILAADESVGSMAKRLSQIGVENTEENRRLYRQVLFSADDRVKKCIGGVIFFHETLYQKDDNGVPFVRTIQDKGIVVGIKVDKGVVPLAGTDGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKISERTPSALAILENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACPIKYTPEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEASFNLNAINRCPLPRPWALTFSYGRALQASALNAWRGQRDNAGAATEEFIKRAEVNGLAAQGKYEGSGEDGGAAAQSLYIANHAY

相同性解析による交差性が予測される生物種
Full Name aldolase C, fructose-bisphosphate
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC003613
Gene Symbol Aldolase C
Gene ID (NCBI) 230
RRIDAB_2881519
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP09972
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).

Protocols

Product Specific Protocols
WB protocol for Aldolase C antibody 66120-1-IgDownload protocol
IHC protocol for Aldolase C antibody 66120-1-IgDownload protocol
IF protocol for Aldolase C antibody 66120-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols