CoraLite® Plus 488-conjugated Aldolase C Monoclonal antibody
Aldolase C Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5H7A5
Cat no : CL488-66120
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of CL488-66120 is Aldolase C Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Aldolase C fusion protein Ag6779 相同性解析による交差性が予測される生物種 |
| Full Name | aldolase C, fructose-bisphosphate |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 39 kDa |
| GenBank accession number | BC003613 |
| Gene symbol | Aldolase C |
| Gene ID (NCBI) | 230 |
| RRID | AB_2919288 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Aldolase C antibody CL488-66120 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
