CoraLite®594-conjugated Aldolase C Monoclonal antibody
Aldolase C Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
5H7A5
Cat no : CL594-66120
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of CL594-66120 is Aldolase C Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Aldolase C fusion protein Ag6779 相同性解析による交差性が予測される生物種 |
Full Name | aldolase C, fructose-bisphosphate |
Calculated molecular weight | 39 kDa |
Observed molecular weight | 39 kDa |
GenBank accession number | BC003613 |
Gene symbol | Aldolase C |
Gene ID (NCBI) | 230 |
RRID | AB_2919950 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).
Protocols
Product Specific Protocols | |
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IF protocol for CL594 Aldolase C antibody CL594-66120 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |