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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated Aldolase C Monoclonal anti (CL594-66120)

    IF Staining of HepG2 using CL594-66120

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Aldolase C antibody (CL594-66120, Clone: 5H7A5 ) at dilution of 1:200.

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CoraLite®594-conjugated Aldolase C Monoclonal antibody

Aldolase C Monoclonal Antibody for IF/ICC
Cat No. CL594-66120
Clone No.5H7A5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

ALDOC, 5H7A5, ALDC, ALDO, Brain-type aldolase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66120 targets Aldolase C in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Aldolase C fusion protein Ag6779 相同性解析による交差性が予測される生物種
Full Name aldolase C, fructose-bisphosphate
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC003613
Gene Symbol Aldolase C
Gene ID (NCBI) 230
RRIDAB_2919950
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09972
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).

Protocols

Product Specific Protocols
IF protocol for CL594 Aldolase C antibody CL594-66120Download protocol
Standard Protocols
Click here to view our Standard Protocols