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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Alpha 1B-Glycoprotein Monoclonal antibody

Alpha 1B-Glycoprotein Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4D4E5

Cat no : CL594-66260

Synonyms

A1B, A1BG, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein, DKFZp686F0970, GAB, HYST2477

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated Alpha 1B-Glycoprotein Mono (CL594-66260)

    IF Staining of HepG2 using CL594-66260

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Alpha 1B-Glycoprotein antibody (CL594-66260, Clone: 4D4E5 ) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66260 targets Alpha 1B-Glycoprotein in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alpha 1B-Glycoprotein fusion protein Ag5414 相同性解析による交差性が予測される生物種
Full Name alpha-1-B glycoprotein
Calculated molecular weight 54 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC035719
Gene symbol A1BG
Gene ID (NCBI) 1
RRIDAB_2919973
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).

Protocols

Product Specific Protocols
IF protocol for CL594 Alpha 1B-Glycoprotein antibody CL594-66260Download protocol
Standard Protocols
Click here to view our Standard Protocols