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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha-2-antiplasmin Polyclonal antibody (28549-1-AP)

    WB analysis using 28549-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using Alpha-2-antiplasmin Polyclonal antibody (28549-1-AP)

    WB analysis of human plasma using 28549-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Alpha-2-antiplasmin Polyclonal antibody (28549-1-AP)

    IHC staining of mouse liver using 28549-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Alpha-2-antiplasmin Polyclonal antibody (28549-1-AP)

    IHC staining of mouse liver using 28549-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha 2-Antiplasmin Polyclonal antibody (28549-1-AP)

    IF Staining of HepG2 using 28549-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha-2-antiplasmin antibody (28549-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Alpha 2-Antiplasmin Polyclonal antibody

Alpha 2-Antiplasmin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28549-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Alpha-2-antiplasmin, Serpin F2, ALPHA 2 PI, Alpha 2 AP, Alpha 2 antiplasmin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, THP-1 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28549-1-AP targets Alpha 2-Antiplasmin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha 2-Antiplasmin fusion protein Ag29670 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
Calculated molecular weight 491 aa, 55 kDa
Observed molecular weight55 kDa
GenBank accession numberBC031592
Gene Symbol Alpha-2-antiplasmin
Gene ID (NCBI) 5345
RRIDAB_2918174
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08697
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERPINF2, also termed Alpha-2-antiplasmin(a2-AP), a member of the serpin family of serine protease inhibitors. SERPINF2 is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in SERPINF2 result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis.

Protocols

Product Specific Protocols
WB protocol for Alpha 2-Antiplasmin antibody 28549-1-APDownload protocol
IHC protocol for Alpha 2-Antiplasmin antibody 28549-1-APDownload protocol
IF protocol for Alpha 2-Antiplasmin antibody 28549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols