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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha-2-antiplasmin Polyclonal antibody

Alpha-2-antiplasmin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28549-1-AP

Synonyms

a2-antiplasmin, A2AP, AAP, Alpha 2 antiplasmin, Alpha 2 AP, ALPHA 2 PI, Alpha 2 plasmin inhibitor, Alpha-2-antiplasmin, API, PLI, Serpin F2, SERPINF2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha-2-antiplasmin Polyclonal antibody (28549-1-AP)

    WB analysis using 28549-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using Alpha-2-antiplasmin Polyclonal antibody (28549-1-AP)

    WB analysis of human plasma using 28549-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Alpha-2-antiplasmin Polyclonal antibody (28549-1-AP)

    IHC staining of mouse liver using 28549-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Alpha-2-antiplasmin Polyclonal antibody (28549-1-AP)

    IHC staining of mouse liver using 28549-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28549-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha-2-antiplasmin Polyclonal antibody (28549-1-AP)

    IF Staining of HepG2 using 28549-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha-2-antiplasmin antibody (28549-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, THP-1 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28549-1-AP targets Alpha-2-antiplasmin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha-2-antiplasmin fusion protein Ag29670 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
Calculated molecular weight 491 aa, 55 kDa
Observed molecular weight55 kDa
GenBank accession numberBC031592
Gene symbol Alpha-2-antiplasmin
Gene ID (NCBI) 5345
RRIDAB_2918174
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERPINF2, also termed Alpha-2-antiplasmin(a2-AP), a member of the serpin family of serine protease inhibitors. SERPINF2 is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in SERPINF2 result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis.

Protocols

Product Specific Protocols
WB protocol for Alpha-2-antiplasmin antibody 28549-1-APDownload protocol
IHC protocol for Alpha-2-antiplasmin antibody 28549-1-APDownload protocol
IF protocol for Alpha-2-antiplasmin antibody 28549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols