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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Alpha-2-antiplasmin Polyclonal antibody

Alpha-2-antiplasmin Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28549

Synonyms

Alpha 2-Antiplasmin, Serpin F2, ALPHA 2 PI, Alpha 2 AP, Alpha 2 antiplasmin

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated Alpha-2-antiplasmin (CL488-28549)

    IF Staining of HepG2 using CL488-28549

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Alpha-2-antiplasmin antibody (CL488-28549) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated Alpha-2-antiplasmin (CL488-28549)

    FC experiment of HepG2 using CL488-28549

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Alpha-2-Antiplasmin Polyclonal Antibody (CL488-28549)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28549 targets Alpha-2-antiplasmin in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha-2-antiplasmin fusion protein Ag29670 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
Calculated molecular weight 491 aa, 55 kDa
Observed molecular weight55 kDa
GenBank accession numberBC031592
Gene symbol Alpha-2-antiplasmin
Gene ID (NCBI) 5345
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERPINF2, also termed Alpha-2-antiplasmin(a2-AP), a member of the serpin family of serine protease inhibitors. SERPINF2 is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in SERPINF2 result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Alpha-2-antiplasmin antibody CL488-28549Download protocol
FC protocol for CL Plus 488 Alpha-2-antiplasmin antibody CL488-28549Download protocol
Standard Protocols
Click here to view our Standard Protocols