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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha-2-Macroglobulin Recombinant antibody, PBS Only

Alpha-2-Macroglobulin Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241649D2

Cat no : 84347-5-PBS

Synonyms

A2M, 241649D2, Alpha-2-M, Alpha 2M, alpha 2 macroglobulin

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Alpha-2-Macroglobulin Recombinant antibody (84347-5-RR)

    WB analysis of human plasma using 84347-5-RR (same clone as 84347-5-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 84347-5-RR (Alpha-2-Macroglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84347-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha-2-Macroglobulin Recombinant antibody (84347-5-RR)

    IF Staining of HepG2 using 84347-5-RR (same clone as 84347-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (84347-5-RR, Clone: 241649D2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84347-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Alpha-2-Macroglobulin Recombinant antibody (84347-5-RR)

    FC experiment of HepG2 using 84347-5-RR (same clone as 84347-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Alpha-2-Macroglobulin Recombinant antibody (84347-5-RR, Clone:241649D2) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84347-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84347-5-RR

    Affinity and Kinetic Characterization of 84347-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84347-5-RR against Human Alpha-2-Macroglobulin were performed. The affinity constant is 0.193 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84347-5-PBS is Alpha-2-Macroglobulin Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name alpha-2-macroglobulin
Calculated molecular weight163kDa
Observed molecular weight 185 kDa
GenBank accession numberNM_000014.6
Gene symbol Alpha 2 Macroglobulin
Gene ID (NCBI) 2
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Alpha-2-Macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.