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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Alpha-2-Macroglobulin Recombinant antibody (84347-5-RR)

    WB analysis of human plasma using 84347-5-RR

    human plasma were subjected to SDS PAGE followed by western blot with 84347-5-RR (Alpha-2-Macroglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha-2-Macroglobulin Recombinant antibody (84347-5-RR)

    IF Staining of HepG2 using 84347-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (84347-5-RR, Clone: 241649D2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using Alpha-2-Macroglobulin Recombinant antibody (84347-5-RR)

    FC experiment of HepG2 using 84347-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Alpha-2-Macroglobulin Recombinant antibody (84347-5-RR, Clone:241649D2) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84347-5-RR

    Affinity and Kinetic Characterization of 84347-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84347-5-RR against Human Alpha-2-Macroglobulin were performed. The affinity constant is 0.193 nM.

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Alpha-2-Macroglobulin Recombinant antibody

Alpha-2-Macroglobulin Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84347-5-RR
Clone No.241649D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

A2M, 241649D2, Alpha-2-M, Alpha 2M, alpha 2 macroglobulin

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman plasma
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84347-5-RR targets Alpha-2-Macroglobulin in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name alpha-2-macroglobulin
Calculated molecular weight163kDa
Observed molecular weight 185 kDa
GenBank accession numberNM_000014.6
Gene Symbol Alpha 2 Macroglobulin
Gene ID (NCBI) 2
RRIDAB_3671888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha-2-Macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.

Protocols

Product Specific Protocols
WB protocol for Alpha-2-Macroglobulin antibody 84347-5-RRDownload protocol
IF protocol for Alpha-2-Macroglobulin antibody 84347-5-RRDownload protocol
FC protocol for Alpha-2-Macroglobulin antibody 84347-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols