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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated Alpha-2-Macroglobulin Mono (CL594-66126)

    IF Staining of HepG2 using CL594-66126

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66126 (Alpha-2-macroglobulin antibody) at dilution of 1:100.

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CoraLite®594-conjugated Alpha-2-Macroglobulin Monoclonal antibody

Alpha-2-Macroglobulin Monoclonal Antibody for IF/ICC
Cat No. CL594-66126
Clone No.4B11F7

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Synonyms

A2M, Alpha 2 M, alpha 2 macroglobulin, CPAMD5, DKFZp779B086, FWP007, S863 7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66126 targets Alpha-2-Macroglobulin in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Alpha-2-Macroglobulin fusion protein Ag19245 相同性解析による交差性が予測される生物種
Full Name alpha-2-macroglobulin
Calculated molecular weight 1474 aa, 163 kDa
GenBank accession numberBC040071
Gene Symbol Alpha 2 Macroglobulin
Gene ID (NCBI) 2
RRIDAB_2883520
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01023
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.

Protocols

Product Specific Protocols
IF protocol for CL594 Alpha-2-Macroglobulin antibody CL594-66126Download protocol
Standard Protocols
Click here to view our Standard Protocols