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Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®594-conjugated Alpha E-Catenin Monoclonal (CL594-66221)

    FC experiment of MCF-7 using CL594-66221

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Alpha E-Catenin (CL594-66221, Clone:2B7B8) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite®594-conjugated Alpha E-Catenin Monoclonal antibody

Alpha E-Catenin Monoclonal Antibody for FC (Intra)
Cat No. CL594-66221
Clone No.2B7B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC (Intra)

Synonyms

Alpha E catenin, Alpha E-Catenin, Cadherin associated protein, CAP102, Catenin alpha 1, CTNNA1, FLJ36832, FLJ52416

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66221 targets Alpha E-Catenin in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alpha E-Catenin fusion protein Ag23603 相同性解析による交差性が予測される生物種
Full Name catenin (cadherin-associated protein), alpha 1, 102kDa
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 95-100 kDa
GenBank accession numberBC031262
Gene Symbol Alpha E-Catenin
Gene ID (NCBI) 1495
RRIDAB_3084704
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35221
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.

Protocols

Product Specific Protocols
FC protocol for CL594 Alpha E-Catenin antibody CL594-66221Download protocol
Standard Protocols
Click here to view our Standard Protocols