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  • Featured Product
  • KD/KO Validated

Alpha Tubulin Monoclonal antibody, PBS Only

Alpha Tubulin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

1E4C11

Cat no : 66031-1-PBS

Synonyms

TUBA1B, Tubulin alpha 1B chain, Tubulin alpha, EC:3.6.5.-, Detyrosinated tubulin alpha-1B chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    WB analysis using 66031-1-Ig (same clone as 66031-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    WB analysis of HeLa using 66031-1-Ig (same clone as 66031-1-PBS)

    WB result of Alpha Tubulin antibody (66031-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Tubulin transfected HeLa cells. This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IP experiment of HeLa using 66031-1-Ig (same clone as 66031-1-PBS)

    IP result of anti-Alpha Tubulin (IP:66031-1-Ig, 5ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human tonsillitis using 66031-1-Ig (same clone as 66031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human colon cancer using 66031-1-Ig (same clone as 66031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human colon cancer using 66031-1-Ig (same clone as 66031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human liver cancer using 66031-1-Ig (same clone as 66031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human tonsillitis using 66031-1-Ig (same clone as 66031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of HepG2 using 66031-1-Ig (same clone as 66031-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of HeLa using 66031-1-Ig (same clone as 66031-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Histone H1.2 antibody (19649-1-AP, green). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of MCF-7 using 66031-1-Ig (same clone as 66031-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), ZO-1 antibody (21773-1-AP, green). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of MCF-7 using 66031-1-Ig (same clone as 66031-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ZO-1 antibody (21773-1-AP, red). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of HepG2 using 66031-1-Ig (same clone as 66031-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    FC experiment of HeLa using 66031-1-Ig (same clone as 66031-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Alpha Tubulin (66031-1-Ig, Clone:1E4C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66031-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66031-1-PBS targets Alpha Tubulin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha Tubulin fusion protein Ag18034 相同性解析による交差性が予測される生物種
Full Name tubulin, alpha 1b
Calculated molecular weight 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC009314
Gene symbol Alpha Tubulin
Gene ID (NCBI) 10376
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

What is the function of alpha tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.

 Where is alpha-tubulin expressed?

Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton. 

What are the post-translational modifications of alpha tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination1, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3  Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).

3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).