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HRP-conjugated Alpha Tubulin Recombinant antibody
Alpha Tubulin Recombinant Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB
Conjugate
HRP
CloneNo.
1G7
Cat no : HRP-80762
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
HRP-80762 targets Alpha Tubulin in WB applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Alpha Tubulin fusion protein Ag18034 相同性解析による交差性が予測される生物種 |
| Full Name | tubulin, alpha 1b |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC009314 |
| Gene symbol | Alpha Tubulin |
| Gene ID (NCBI) | 10376 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP Alpha Tubulin antibody HRP-80762 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
