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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Angiopoietin 1 Monoc (CL488-68618)

    IF Staining of HeLa using CL488-68618

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Angiopoietin 1 antibody (CL488-68618, Clone: 3C4A3 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated Angiopoietin 1 Monoclonal antibody

Angiopoietin 1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68618
Clone No.3C4A3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68618 targets Angiopoietin 1 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Angiopoietin 1 fusion protein Ag18829 相同性解析による交差性が予測される生物種
Full Name angiopoietin 1
Calculated molecular weight 498 aa, 58 kDa
Observed molecular weight70 kDa, 55 kDa
GenBank accession numberBC152411
Gene Symbol Angiopoietin 1
Gene ID (NCBI) 284
RRIDAB_3673026
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15389
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Angiopoietin 1 antibody CL488-68618Download protocol
Standard Protocols
Click here to view our Standard Protocols