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CoraLite®594-conjugated Annexin A1 Monoclonal antibody
Annexin A1 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1E1B7
Cat no : CL594-66344
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | A549 cells |
Positive FC (Intra) detected in | K-562 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-66344 targets Annexin A1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Annexin A1 fusion protein Ag17273 相同性解析による交差性が予測される生物種 |
Full Name | annexin A1 |
Calculated molecular weight | 346 aa, 39 kDa |
Observed molecular weight | 35 kDa |
GenBank accession number | BC001275 |
Gene symbol | Annexin A1 |
Gene ID (NCBI) | 301 |
RRID | AB_2883563 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 Annexin A1 antibody CL594-66344 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |