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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Annexin A11 Monoclonal antibody

Annexin A11 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A3C4

Cat no : 68089-1-Ig

Synonyms

ANXA11, ANX11, Annexin-11, Annexin XI, Annexin 11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Annexin A11 Monoclonal antibody (68089-1-Ig)

    WB analysis using 68089-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68089-1-Ig (Annexin A11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Annexin A11 Monoclonal antibody (68089-1-Ig)

    IHC staining of human lung cancer using 68089-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Annexin A11 Monoclonal antibody (68089-1-Ig)

    IHC staining of human lung cancer using 68089-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Annexin A11 Monoclonal antibody (68089-1-Ig)

    IHC staining of human ovary tumor using 68089-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Annexin A11 Monoclonal antibody (68089-1-Ig)

    IHC staining of human ovary tumor using 68089-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Annexin A11 Monoclonal antibody (68089-1-Ig)

    IF Staining of HeLa using 68089-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A11 antibody (68089-1-Ig, Clone: 1A3C4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293T cells using Annexin A11 Monoclonal antibody (68089-1-Ig)

    FC experiment of HEK-293T using 68089-1-Ig

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human Annexin A11 (68089-1-Ig, Clone:1A3C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using Annexin A11 Monoclonal antibody (68089-1-Ig)

    FC experiment of HeLa using 68089-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Annexin A11 (68089-1-Ig, Clone:1A3C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA431 cells, A549 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig heart tissue, rabbit heart
Positive IHC detected inhuman lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293T cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68089-1-Ig targets Annexin A11 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Annexin A11 fusion protein Ag31839 相同性解析による交差性が予測される生物種
Full Name annexin A11
Calculated molecular weight 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC007564
Gene symbol Annexin A11
Gene ID (NCBI) 311
RRIDAB_2918826
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling. Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer.

Protocols

Product Specific Protocols
WB protocol for Annexin A11 antibody 68089-1-IgDownload protocol
IHC protocol for Annexin A11 antibody 68089-1-IgDownload protocol
IF protocol for Annexin A11 antibody 68089-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell Proteomics

FMRP long-range transport and degradation are mediated by Dynlrb1 in sensory neurons

Authors - Sara Emad El-Agamy
View Article
humanWB,IHC

Acta Neuropathol

Annexin A11 aggregation in FTLD-TDP type C and related neurodegenerative disease proteinopathies

Authors - John L Robinson
View Article