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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Annexin A11 Monoclonal antibody (68089-1-Ig)

    WB analysis using 68089-1-Ig (same clone as 68089-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68089-1-Ig (Annexin A11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Annexin A11 Monoclonal antibody (68089-1-Ig)

    IHC staining of human lung cancer using 68089-1-Ig (same clone as 68089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Annexin A11 Monoclonal antibody (68089-1-Ig)

    IHC staining of human lung cancer using 68089-1-Ig (same clone as 68089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Annexin A11 Monoclonal antibody (68089-1-Ig)

    IHC staining of human ovary tumor using 68089-1-Ig (same clone as 68089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Annexin A11 Monoclonal antibody (68089-1-Ig)

    IHC staining of human ovary tumor using 68089-1-Ig (same clone as 68089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68089-1-Ig (Annexin A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Annexin A11 Monoclonal antibody (68089-1-Ig)

    IF Staining of HeLa using 68089-1-Ig (same clone as 68089-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A11 antibody (68089-1-Ig, Clone: 1A3C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293T cells using Annexin A11 Monoclonal antibody (68089-1-Ig)

    FC experiment of HEK-293T using 68089-1-Ig (same clone as 68089-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human Annexin A11 (68089-1-Ig, Clone:1A3C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Annexin A11 Monoclonal antibody (68089-1-Ig)

    FC experiment of HeLa using 68089-1-Ig (same clone as 68089-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Annexin A11 (68089-1-Ig, Clone:1A3C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68089-1-PBS in a different storage buffer formulation.

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Annexin A11 Monoclonal antibody, PBS Only

Annexin A11 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 68089-1-PBS
Clone No.1A3C4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

ANXA11, ANX11, Annexin-11, Annexin XI, Annexin 11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68089-1-PBS targets Annexin A11 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Annexin A11 fusion protein Ag31839 相同性解析による交差性が予測される生物種
Full Name annexin A11
Calculated molecular weight 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC007564
Gene Symbol Annexin A11
Gene ID (NCBI) 311
RRIDAB_2918826
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50995
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling (PMID: 31610865). ANXA11, an RNA granule-associated phosphoinositide-binding protein, acts as a molecular tether between RNA granules and lysosomes (PMID: 31539493). Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer. Annexin A11, is involved in a variety of cellular functions including mRNA transport and translation, endocytosis, exocytosis, and plasma membrane repair (PMID: 38896345).