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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Apolipoprotein AI Monoclonal antibody

Apolipoprotein AI Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C9G5

Cat no : CL594-66206

Synonyms

APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated Apolipoprotein AI Monoclon (CL594-66206)

    IF Staining of HepG2 using CL594-66206

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66206 (Apolipoprotein AI antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using CoraLite®594-conjugated Apolipoprotein AI Monoclon (CL594-66206)

    IF Staining of L02 using CL594-66206

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using CoraLite®594-conjugated Apolipoprotein AI antibody (CL594-66206, Clone: 1C9G5 ) at dilution of 1:100, CL488-Phalloidin (green).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated Apolipoprotein AI Monoclon (CL594-66206)

    FC experiment of HepG2 using CL594-66206

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Apolipoprotein AI (CL594-66206, Clone:1C9G5) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells, L02 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66206 targets Apolipoprotein AI in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Apolipoprotein AI fusion protein Ag21920 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-I
Calculated molecular weight 31 kDa
Observed molecular weight 26-28 kDa
GenBank accession numberBC005380
Gene symbol APOA1
Gene ID (NCBI) 335
RRIDAB_2883535
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protocols

Product Specific Protocols
IF protocol for CL594 Apolipoprotein AI antibody CL594-66206Download protocol
Standard Protocols
Click here to view our Standard Protocols