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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse Apolipoprotein E Recombinant antibody, PBS Only

Apolipoprotein E Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240786C3

Cat no : 83728-4-PBS

Synonyms

Apoe, AI255918, 240786C3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Apolipoprotein E Recombinant antibody (83728-4-RR)

    WB analysis using 83728-4-RR (same clone as 83728-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Apolipoprotein E Recombinant antibody (83728-4-RR)

    IF Staining of HepG2 using 83728-4-RR (same clone as 83728-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein E antibody (83728-4-RR, Clone: 240786C3 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83728-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83728-4-RR

    Affinity and Kinetic Characterization of 83728-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83728-4-RR against Mouse Apolipoprotein E were performed. The affinity constant is 0.793 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83728-4-PBS is Apolipoprotein E Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name Apolipoprotein E
Calculated molecular weight36kDa
Observed molecular weight36 kDa
GenBank accession numberNM_001305819.1
Gene symbol Apolipoprotein E
Gene ID (NCBI) 11816
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Apolipoprotein E (apoE) is a core component of plasma lipoproteins that participates in diverse biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immunoregulation, and neuronal growth and repair (PMID: 17854398). Mouse apoE, like apoE4, contains the equivalent of Arg-112 and Glu-255, but lacks the critical Arg-61 equivalent (it contains Thr-61). Thus, mouse apoE does not display apoE4 domain interaction (PMID: 11553788).