• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Apolipoprotein E Recombinant antibody

Apolipoprotein E Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240786C3

Cat no : 83728-4-RR

Synonyms

Apoe, AI255918, 240786C3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Apolipoprotein E Recombinant antibody (83728-4-RR)

    WB analysis using 83728-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83728-4-RR (Apolipoprotein E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Apolipoprotein E Recombinant antibody (83728-4-RR)

    IF Staining of HepG2 using 83728-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein E antibody (83728-4-RR, Clone: 240786C3 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83728-4-RR

    Affinity and Kinetic Characterization of 83728-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83728-4-RR against Mouse Apolipoprotein E were performed. The affinity constant is 0.793 nM.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inmouse liver tissue, mouse serum, mouse brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83728-4-RR is Apolipoprotein E Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name Apolipoprotein E
Calculated molecular weight36kDa
Observed molecular weight36 kDa
GenBank accession numberNM_001305819.1
Gene symbol Apolipoprotein E
Gene ID (NCBI) 11816
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apolipoprotein E (apoE) is a core component of plasma lipoproteins that participates in diverse biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immunoregulation, and neuronal growth and repair (PMID: 17854398). Mouse apoE, like apoE4, contains the equivalent of Arg-112 and Glu-255, but lacks the critical Arg-61 equivalent (it contains Thr-61). Thus, mouse apoE does not display apoE4 domain interaction (PMID: 11553788).

Protocols

Product Specific Protocols
WB protocol for Apolipoprotein E antibody 83728-4-RRDownload protocol
IF protocol for Apolipoprotein E antibody 83728-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols