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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite® Plus 488-conjugated Apolipoprotein H Mon (CL488-66074)

    IF Staining of human liver cancer using CL488-66074

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 Apolipoprotein H antibody (CL488-66074, Clone: 4D9A4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated Apolipoprotein H Mon (CL488-66074)

    IF Staining of HepG2 using CL488-66074

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Apolipoprotein H antibody (CL488-66074, Clone: 4D9A4 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated APOH Monoclonal antibody

APOH Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-66074
Clone No.4D9A4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, IF-P

Synonyms

Apolipoprotein H, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66074 targets APOH in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen APOH fusion protein Ag17915 相同性解析による交差性が予測される生物種
Full Name apolipoprotein H (beta-2-glycoprotein I)
Calculated molecular weight 345 aa, 38 kDa
Observed molecular weight 50-53 kDa
GenBank accession numberBC026283
Gene Symbol APOH
Gene ID (NCBI) 350
RRIDAB_2919283
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02749
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 APOH antibody CL488-66074Download protocol
Standard Protocols
Click here to view our Standard Protocols