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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Aquaporin 4 Polyclonal antibody

Aquaporin 4 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-16473

Synonyms

AQP 4, AQP4, aquaporin 4, HMIWC2, MIWC, WCH4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using CoraLite®594-conjugated Aquaporin 4 Polyclonal ant (CL594-16473)

    IF Staining of rat brain using CL594-16473

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 Aquaporin 4 antibody (CL594-16473) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated Aquaporin 4 Polyclonal ant (CL594-16473)

    IF Staining of mouse brain using CL594-16473

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Aquaporin 4 antibody (CL594-16473) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inrat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-16473 is Aquaporin 4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aquaporin 4 fusion protein Ag9561 相同性解析による交差性が予測される生物種
Full Name Aquaporin 4
Calculated molecular weight 323 aa, 35 kDa
Observed molecular weight 35-37 kDa, 32-34 kDa
GenBank accession numberBC022286
Gene symbol Aquaporin 4
Gene ID (NCBI) 361
RRIDAB_2919831
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

Protocols

Product Specific Protocols
IF protocol for CL594 Aquaporin 4 antibody CL594-16473Download protocol
Standard Protocols
Click here to view our Standard Protocols