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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Beta Arrestin 1 Recombinant antibody, PBS Only (Detector)

Beta Arrestin 1 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240974C3

Cat no : 83837-1-PBS

Synonyms

ARRB1, ARR1, ARR 1, ARB2, ARB1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Beta Arrestin 1 Recombinant antibody (83837-1-RR)

    WB analysis using 83837-1-RR (same clone as 83837-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83837-1-RR (Beta Arrestin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83837-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Beta Arrestin 1 Recombinant antibody (83837-1-RR)

    WB analysis using 83837-1-RR (same clone as 83837-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83837-1-RR (Arrestin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83837-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Beta Arrestin 1 Recombinant antibody (83837-1-RR)

    WB analysis using 83837-1-RR (same clone as 83837-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 83837-1-RR (Arrestin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83837-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00811-2

    Cytometric bead array standard curve of MP00811-2

    Cytometric bead array standard curve of MP00811-2, Arrestin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83837-2-PBS. Detection antibody: 83837-1-PBS. Standard: Ag7608. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83837-1-RR

    Affinity and Kinetic Characterization of 83837-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83837-1-RR against Human Beta Arrestin 1 were performed. The affinity constant is 0.759 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83837-1-PBS targets Beta Arrestin 1 as part of a matched antibody pair:

MP00811-2: 83837-2-PBS capture and 83837-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Beta Arrestin 1 fusion protein Ag7608 相同性解析による交差性が予測される生物種
Full Name arrestin, beta 1
Calculated molecular weight 47 kDa
Observed molecular weight47-55 kDa
GenBank accession numberBC003636
Gene symbol Beta Arrestin 1
Gene ID (NCBI) 408
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).