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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta Arrestin 1 Recombinant antibody (83837-1-RR)

    WB analysis using 83837-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83837-1-RR (Beta Arrestin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Beta Arrestin 1 Recombinant antibody (83837-1-RR)

    WB analysis using 83837-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83837-1-RR (Arrestin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Beta Arrestin 1 Recombinant antibody (83837-1-RR)

    WB analysis using 83837-1-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 83837-1-RR (Arrestin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83837-1-RR

    Affinity and Kinetic Characterization of 83837-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83837-1-RR against Human Beta Arrestin 1 were performed. The affinity constant is 0.759 nM.

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Beta Arrestin 1 Recombinant antibody

Beta Arrestin 1 Recombinant Antibody for WB, ELISA
Cat No. 83837-1-RR
Clone No.240974C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ARRB1, ARR1, ARR 1, ARB2, ARB1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, RAW 264.7 cells, mouse brain tissue, rat lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83837-1-RR targets Beta Arrestin 1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Beta Arrestin 1 fusion protein Ag7608 相同性解析による交差性が予測される生物種
Full Name arrestin, beta 1
Calculated molecular weight 47 kDa
Observed molecular weight47-55 kDa
GenBank accession numberBC003636
Gene Symbol Beta Arrestin 1
Gene ID (NCBI) 408
RRIDAB_3671420
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49407
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).

Protocols

Product Specific Protocols
WB protocol for Beta Arrestin 1 antibody 83837-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols