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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Beta-2-Microglobulin Polyclonal antibody

Beta-2-Microglobulin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13511-1-AP

Synonyms

B2M, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3, beta 2 microglobulin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    WB analysis using 13511-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    WB analysis using 13511-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    WB analysis using 13511-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    WB analysis of human heart using 13511-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human stomach tissue using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    WB analysis of human stomach using 13511-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    WB analysis using 13511-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    IP experiment of A431 using 13511-1-AP

    IP result of anti-Beta-2-Microglobulin (IP:13511-1-AP, 4ug; Detection:13511-1-AP 1:600) with A431 cells lysate 2280 ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    IHC staining of human tonsillitis using 13511-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    IHC staining of human tonsillitis using 13511-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    IHC staining of human prostate cancer using 13511-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    IHC staining of human prostate cancer using 13511-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    IHC staining of human oesophagus cancer using 13511-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    IHC staining of human oesophagus cancer using 13511-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    IHC staining of mouse lung using 13511-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    IHC staining of mouse lung using 13511-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    IHC staining of human liver cancer using 13511-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    IHC staining of human liver cancer using 13511-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    IF Staining of A431 using 13511-1-AP

    Immunofluorescent analysis of A431 cells using Beta-2-Microglobulin antibody (13511-1-AP) at dilution of 1:750 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using Beta-2-Microglobulin Polyclonal antibody (13511-1-AP)

    IF Staining of NCCIT using 13511-1-AP

    Immunofluorescent analysis of NCCIT cells using Beta-2-Microglobulin antibody (13511-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, human heart tissue, human stomach tissue, mouse lung tissue, HepG2 cells, Raji cells, mouse spleen tissue, rat spleen tissue, HeLa cells, Jurkat cells, rat lung tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman tonsillitis tissue, mouse lung tissue, human liver cancer tissue, human prostate cancer tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, NCCIT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:375-1:1500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13511-1-AP targets Beta-2-Microglobulin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta-2-Microglobulin fusion protein Ag4433 相同性解析による交差性が予測される生物種
Full Name beta-2-microglobulin
Calculated molecular weight 119 aa, 14 kDa
Observed molecular weight 12-14 kDa
GenBank accession numberBC032589
Gene symbol B2M
Gene ID (NCBI) 567
RRIDAB_2062735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Protocols

Product Specific Protocols
WB protocol for Beta-2-Microglobulin antibody 13511-1-APDownload protocol
IHC protocol for Beta-2-Microglobulin antibody 13511-1-APDownload protocol
IF protocol for Beta-2-Microglobulin antibody 13511-1-APDownload protocol
IP protocol for Beta-2-Microglobulin antibody 13511-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

IRGQ-mediated autophagy in MHC class I quality control promotes tumor immune evasion

Authors - Lina Herhaus
View Article
humanIF

Sci Adv

Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.

Authors - Yuan Wang
View Article

Nat Commun

Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology.

Authors - Kyota Fujita
View Article
mouseWB

Theranostics

Nintedanib enhances the efficacy of PD-L1 blockade by upregulating MHC-I and PD-L1 expression in tumor cells.

Authors - Jingyao Tu
View Article
IP

Biomater Sci

Enhanced β2-microglobulin binding of a "navigator" molecule bearing a single-chain variable fragment antibody for artificial switching of metabolic processing pathways.

Authors - Yusuke Kambe
View Article
ratIHC

Aging (Albany NY)

β2-microglobulin as a biomarker of pulmonary fibrosis development in COPD patients.

Authors - Zhenchao Wu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Cali (Verified Customer) (10-24-2023)

A reliable Ab to detect endogenous B2M.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
Beta-2-Microglobulin Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:13511-1-AP)