Beta-2-Microglobulin Polyclonal antibody

Name: Beta-2-Microglobulin Polyclonal antibody
Brand: Proteintech
SKU: 13511-1-AP
Rating: 5.00 (1 reviews)

Beta-2-Microglobulin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 13511-1-AP

Synonyms

B2M, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3, beta 2 microglobulin

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 13511-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 13511-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 13511-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 13511-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 13511-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human stomach using 13511-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 13511-1-AP
IP result of anti-Beta-2-Microglobulin (IP:13511-1-AP, 4ug; Detection:13511-1-AP 1:600) with A431 cells lysate 2280 ug.
IHC staining of human tonsillitis using 13511-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 13511-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 13511-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 13511-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 13511-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 13511-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 13511-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 13511-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13511-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13511-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 13511-1-AP
Immunofluorescent analysis of A431 cells using Beta-2-Microglobulin antibody (13511-1-AP) at dilution of 1:750 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of NCCIT using 13511-1-AP
Immunofluorescent analysis of NCCIT cells using Beta-2-Microglobulin antibody (13511-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 13511-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, human heart tissue, human stomach tissue, mouse lung tissue, HepG2 cells, Raji cells, mouse spleen tissue, rat spleen tissue, HeLa cells, Jurkat cells, rat lung tissue
Positive IP detected in	A431 cells
Positive IHC detected in	human tonsillitis tissue, mouse lung tissue, human liver cancer tissue, human prostate cancer tissue, human oesophagus cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells, NCCIT cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICC	IF/ICC : 1:375-1:1500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 11 publications below
IHC	See 5 publications below
IF	See 6 publications below
IP	See 1 publications below

Product Information

13511-1-AP targets Beta-2-Microglobulin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Beta-2-Microglobulin fusion protein Ag4433 相同性解析による交差性が予測される生物種
Full Name	beta-2-microglobulin
Calculated molecular weight	119 aa, 14 kDa
Observed molecular weight	12-14 kDa
GenBank accession number	BC032589
Gene symbol	B2M
Gene ID (NCBI)	567
RRID	AB_2062735
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Protocols

Product Specific Protocols
WB protocol for Beta-2-Microglobulin antibody 13511-1-AP	Download protocol
IHC protocol for Beta-2-Microglobulin antibody 13511-1-AP	Download protocol
IF protocol for Beta-2-Microglobulin antibody 13511-1-AP	Download protocol
IP protocol for Beta-2-Microglobulin antibody 13511-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Sci Adv Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor. Authors - Yuan Wang View Article
		Nat Commun Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology. Authors - Kyota Fujita View Article
mouse	WB	Theranostics Nintedanib enhances the efficacy of PD-L1 blockade by upregulating MHC-I and PD-L1 expression in tumor cells. Authors - Jingyao Tu View Article
	IP	Biomater Sci Enhanced β2-microglobulin binding of a "navigator" molecule bearing a single-chain variable fragment antibody for artificial switching of metabolic processing pathways. Authors - Yusuke Kambe View Article
rat	IHC	Aging (Albany NY) β2-microglobulin as a biomarker of pulmonary fibrosis development in COPD patients. Authors - Zhenchao Wu View Article
human,mouse	WB,IHC	Immunology B2M gene expression shapes the immune landscape of lung adenocarcinoma and determines the response to immunotherapy. Authors - Yu Zhao View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Cali (Verified Customer) (10-24-2023)

A reliable Ab to detect endogenous B2M.

Applications: Western Blot
Primary Antibody Dilution: 1:2000
Cell Tissue Type: mouse brain lysate

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Beta-2-Microglobulin Polyclonal antibody

Beta-2-Microglobulin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 13511-1-AP

WB analysis using 13511-1-AP

WB analysis using 13511-1-AP

WB analysis using 13511-1-AP

WB analysis of human heart using 13511-1-AP

WB analysis of human stomach using 13511-1-AP

IP experiment of A431 using 13511-1-AP

IHC staining of human tonsillitis using 13511-1-AP

IHC staining of human tonsillitis using 13511-1-AP

IHC staining of human prostate cancer using 13511-1-AP

IHC staining of human prostate cancer using 13511-1-AP

IHC staining of human oesophagus cancer using 13511-1-AP

IHC staining of human oesophagus cancer using 13511-1-AP

IHC staining of mouse lung using 13511-1-AP

IHC staining of mouse lung using 13511-1-AP

IHC staining of human liver cancer using 13511-1-AP

IHC staining of human liver cancer using 13511-1-AP

IF Staining of A431 using 13511-1-AP

IF Staining of NCCIT using 13511-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.

Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology.

Nintedanib enhances the efficacy of PD-L1 blockade by upregulating MHC-I and PD-L1 expression in tumor cells.

Enhanced β2-microglobulin binding of a "navigator" molecule bearing a single-chain variable fragment antibody for artificial switching of metabolic processing pathways.

β2-microglobulin as a biomarker of pulmonary fibrosis development in COPD patients.

B2M gene expression shapes the immune landscape of lung adenocarcinoma and determines the response to immunotherapy.

Reviews

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