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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated Beta-2-Microglobulin Polyclonal antibody

Beta-2-Microglobulin Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13511

Synonyms

B2M, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3, beta 2 microglobulin

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 647-conjugated Beta-2-Microglobulin (CL647-13511)

    IF Staining of A431 using CL647-13511

    Immunofluorescent analysis of (-20°C Acetone) fixed A431 cells using CoraLite® Plus 647 Beta-2-Microglobulin antibody (CL647-13511) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-13511 targets Beta-2-Microglobulin in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta-2-Microglobulin fusion protein Ag4433 相同性解析による交差性が予測される生物種
Full Name beta-2-microglobulin
Calculated molecular weight 119 aa, 14 kDa
Observed molecular weight 12-14 kDa
GenBank accession numberBC032589
Gene symbol B2M
Gene ID (NCBI) 567
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Beta-2-Microglobulin antibody CL647-13511Download protocol
Standard Protocols
Click here to view our Standard Protocols