Beta-2-Microglobulin Monoclonal antibody
Beta-2-Microglobulin Monoclonal Antibody for IF/ICC, FC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF/ICC, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1H3F4
Cat no : 68395-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | NCCIT cells, A431 cells |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
Flow Cytometry (FC) | FC : 1.00 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68395-1-Ig targets Beta-2-Microglobulin in IF/ICC, FC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
Full Name | beta-2-microglobulin |
Calculated molecular weight | 119 aa, 14 kDa |
Observed molecular weight | 12-14 kDa |
GenBank accession number | BC032589 |
Gene symbol | B2M |
Gene ID (NCBI) | 567 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. This antibody can be used for staining living cells.
Protocols
Product Specific Protocols | |
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IF protocol for Beta-2-Microglobulin antibody 68395-1-Ig | Download protocol |
FC protocol for Beta-2-Microglobulin antibody 68395-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |