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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Beta-2-Microglobulin Monoclonal antibody

Beta-2-Microglobulin Monoclonal Antibody for IF/ICC, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1H3F4

Cat no : 68395-1-Ig

Synonyms

B2M, 1H3F4, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using Beta-2-Microglobulin Monoclonal antibody (68395-1-Ig)

    IF Staining of NCCIT using 68395-1-Ig

    Immunofluorescent analysis of NCCIT cells (non-fixation) using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Beta-2-Microglobulin Monoclonal antibody (68395-1-Ig)

    IF Staining of A431 using 68395-1-Ig

    Immunofluorescent analysis of A431 cells using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using Beta-2-Microglobulin Monoclonal antibody (68395-1-Ig)

    FC experiment of Jurkat using 68395-1-Ig

    1x2*10^6 Jurkat cells were surface stained with 1 μg Beta-2-Microglobulin Monoclonal antibody (68395-1-Ig, Clone:1H3F4) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1)(N/A), or 1 μg KLH (66360-1-Ig, Clone: T1F8D3F10) (N/A). Cells were not fixed.

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Tested Applications

Positive IF/ICC detected inNCCIT cells, A431 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC)FC : 1.00 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68395-1-Ig targets Beta-2-Microglobulin in IF/ICC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name beta-2-microglobulin
Calculated molecular weight 119 aa, 14 kDa
Observed molecular weight 12-14 kDa
GenBank accession numberBC032589
Gene symbol B2M
Gene ID (NCBI) 567
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. This antibody can be used for staining living cells.

Protocols

Product Specific Protocols
IF protocol for Beta-2-Microglobulin antibody 68395-1-IgDownload protocol
FC protocol for Beta-2-Microglobulin antibody 68395-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols