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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using CoraLite® Plus 488-conjugated Beta-2-Microglobulin (CL488-68395)

    IF Staining of NCCIT using CL488-68395

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using CoraLite® Plus 488 Beta-2-Microglobulin antibody (CL488-68395, Clone: 1H3F4 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated Beta-2-Microglobulin Monoclonal antibody

Beta-2-Microglobulin Monoclonal Antibody for IF/ICC
Cat No. CL488-68395
Clone No.1H3F4

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

B2M, beta 2 microglobulin, HDCMA22P

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inNCCIT cells

68395-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68395 targets Beta-2-Microglobulin in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name beta-2-microglobulin
Calculated molecular weight 119 aa, 14 kDa
Observed molecular weight 12-14 kDa
GenBank accession numberBC032589
Gene Symbol B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
RRIDAB_3673003
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61769
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. This antibody can be used for staining living cells.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Beta-2-Microglobulin antibody CL488-68395Download protocol
Standard Protocols
Click here to view our Standard Protocols