Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-12807 targets BACE1 in IF/ICC applications and shows reactivity with human, mouse, zebrafish, rat samples.
| Tested Reactivity | human, mouse, zebrafish, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BACE1 fusion protein Ag3559 相同性解析による交差性が予測される生物種 |
| Full Name | beta-site APP-cleaving enzyme 1 |
| Calculated molecular weight | 501 aa, 56 kDa |
| Observed molecular weight | 70-75 kDa, 42-56 kDa |
| GenBank accession number | BC036084 |
| Gene Symbol | BACE1 |
| Gene ID (NCBI) | 23621 |
| RRID | AB_3083931 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P56817 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
beta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, is a neuron-specific membrane-associated protease that participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can be detected with different molecular weight: 42-56kD (prozymogen and isoforms), 65-75 (glycosylated forms) and 160kDa (dimer) (PMID:22741101; 21795680).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 BACE1 antibody CL488-12807 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |