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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BAZ2A Recombinant antibody, PBS Only

BAZ2A Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230205B8

Cat no : 82910-2-PBS

Synonyms

WALp3, TTF I interacting protein 5, TIP5, KIAA0314, hWALp3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAZ2A Recombinant antibody (82910-2-RR)

    WB analysis using 82910-2-RR (same clone as 82910-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82910-2-RR (BAZ2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82910-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using BAZ2A Recombinant antibody (82910-2-RR)

    IF Staining of A549 using 82910-2-RR (same clone as 82910-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAZ2A antibody (82910-2-RR, Clone: 230205B8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82910-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using BAZ2A Recombinant antibody (82910-2-RR)

    IF Staining of U2OS using 82910-2-RR (same clone as 82910-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BAZ2A antibody (82910-2-RR, Clone: 230205B8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82910-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-2 OS using BAZ2A Recombinant antibody (82910-2-RR)

    FC experiment of U-2 OS using 82910-2-RR (same clone as 82910-2-PBS)

    (blue)1x10^6 U-2 OS were intracellularly stained with 0.25 ug Anti-Human BAZ2A (82910-2-RR, Clone:230205B8) and Alexa Fluor™ Plus 647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82910-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82910-2-RR

    Affinity and Kinetic Characterization of 82910-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82910-2-RR against Human BAZ2A were performed. The affinity constant is 1.46 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82910-2-PBS targets BAZ2A in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BAZ2A fusion protein Ag33963 相同性解析による交差性が予測される生物種
Full Name bromodomain adjacent to zinc finger domain, 2A
Calculated molecular weight211 kDa
Observed molecular weight250-270 kDa
GenBank accession numberNM_013449.4
Gene symbol BAZ2A
Gene ID (NCBI) 11176
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BAZ2A (bromodomain adjacent to zinc finger domain 2A), also known as TIP5. It is expected to be located in nucleus, and the protein is expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. BAZ2A is an epigenetic regulator affecting transcription of ribosomal RNA, it is also a regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:28801535). The calculated molecular weight of BAZ2A is 210 kDa.