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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BAZ2A Recombinant antibody

BAZ2A Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230205B8

Cat no : 82910-2-RR

Synonyms

WALp3, TTF I interacting protein 5, TIP5, KIAA0314, hWALp3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAZ2A Recombinant antibody (82910-2-RR)

    WB analysis using 82910-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82910-2-RR (BAZ2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using BAZ2A Recombinant antibody (82910-2-RR)

    IF Staining of A549 using 82910-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAZ2A antibody (82910-2-RR, Clone: 230205B8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using BAZ2A Recombinant antibody (82910-2-RR)

    IF Staining of U2OS using 82910-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BAZ2A antibody (82910-2-RR, Clone: 230205B8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of U-2 OS using BAZ2A Recombinant antibody (82910-2-RR)

    FC experiment of U-2 OS using 82910-2-RR

    (blue)1x10^6 U-2 OS were intracellularly stained with 0.25 ug Anti-Human BAZ2A (82910-2-RR, Clone:230205B8) and Alexa Fluor™ Plus 647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82910-2-RR

    Affinity and Kinetic Characterization of 82910-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82910-2-RR against Human BAZ2A were performed. The affinity constant is 1.46 nM.

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, K-562 cells, MDA-MB-231 cells, PC-3 cells
Positive IF/ICC detected inU2OS cells, A549 cells
Positive FC (Intra) detected inU-2 OS

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82910-2-RR targets BAZ2A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BAZ2A fusion protein Ag33963 相同性解析による交差性が予測される生物種
Full Name bromodomain adjacent to zinc finger domain, 2A
Calculated molecular weight211 kDa
Observed molecular weight250-270 kDa
GenBank accession numberNM_013449.4
Gene symbol BAZ2A
Gene ID (NCBI) 11176
RRIDAB_3670641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAZ2A (bromodomain adjacent to zinc finger domain 2A), also known as TIP5. It is expected to be located in nucleus, and the protein is expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. BAZ2A is an epigenetic regulator affecting transcription of ribosomal RNA, it is also a regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:28801535). The calculated molecular weight of BAZ2A is 210 kDa.

Protocols

Product Specific Protocols
WB protocol for BAZ2A antibody 82910-2-RRDownload protocol
IF protocol for BAZ2A antibody 82910-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols