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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated BAZ2A Recombinant antibody

BAZ2A Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230205B8

Cat no : CL488-82910-2

Synonyms

WALp3, TTF I interacting protein 5, TIP5, KIAA0314, hWALp3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated BAZ2A Recombinant an (CL488-82910-2)

    IF Staining of U2OS using CL488-82910-2

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 BAZ2A antibody (CL488-82910-2, Clone: 230205B8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A549 cells using CoraLite® Plus 488-conjugated BAZ2A Recombinant an (CL488-82910-2)

    FC experiment of A549 using CL488-82910-2

    1x10^6 A549 cells were intracellularly stained with 0.25 ug CoraLite® Plus 488-conjugated BAZ2A Recombinant antibody (CL488-82910-2, Clone:230205B8)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82910-2 targets BAZ2A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BAZ2A fusion protein Ag33963 相同性解析による交差性が予測される生物種
Full Name bromodomain adjacent to zinc finger domain, 2A
Calculated molecular weight211 kDa
Observed molecular weight250-270 kDa
GenBank accession numberNM_013449.4
Gene symbol BAZ2A
Gene ID (NCBI) 11176
RRIDAB_3673111
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAZ2A (bromodomain adjacent to zinc finger domain 2A), also known as TIP5. It is expected to be located in nucleus, and the protein is expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. BAZ2A is an epigenetic regulator affecting transcription of ribosomal RNA, it is also a regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:28801535). The calculated molecular weight of BAZ2A is 210 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 BAZ2A antibody CL488-82910-2Download protocol
FC protocol for CL Plus 488 BAZ2A antibody CL488-82910-2Download protocol
Standard Protocols
Click here to view our Standard Protocols