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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BBS2 Monoclonal antibody

BBS2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, Canine and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E11B3

Cat no : 66246-1-Ig

Synonyms

Bardet Biedl syndrome 2, BBS, BBS2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of K-562 cells using BBS2 Monoclonal antibody (66246-1-Ig)

    WB analysis of K-562 using 66246-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66246-1-Ig (BBS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using BBS2 Monoclonal antibody (66246-1-Ig)

    IF Staining of MDCK using 66246-1-Ig

    Immunofluorescent images of MDCK cells stained with BBS2 mouse mAb (66246-1-Ig) and ARL13B rabbit pAb (17711-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure-Goat anti-Mouse IgG (H+L) for 66246-1-Ig, and Alexa Fluor 594-conjugated Goat Anti-Rabbit IgG (H+L) for 17711-1-AP.

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Tested Applications

Positive WB detected inK-562 cells
Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66246-1-Ig targets BBS2 in WB, IF/ICC, ELISA applications and shows reactivity with human, Canine samples.

Tested Reactivity human, Canine
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen BBS2 fusion protein Ag21390 相同性解析による交差性が予測される生物種
Full Name Bardet-Biedl syndrome 2
Calculated molecular weight 80 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC014140
Gene symbol BBS2
Gene ID (NCBI) 583
RRIDAB_2881635
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BBS2 antibody 66246-1-IgDownload protocol
IF protocol for BBS2 antibody 66246-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

CRISPR/Cas9-mediated genomic editing of Cluap1/IFT38 reveals a new role in actin arrangement.

Authors - Tina Beyer
View Article
mouseWB

J Biol Chem

The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.

Authors - Poppy Datta
View Article