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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BBS4 Recombinant antibody (82961-1-RR)

    WB analysis using 82961-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82961-1-RR (BBS4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BBS4 Recombinant antibody (82961-1-RR)

    WB analysis of HEK-293 using 82961-1-RR

    WB result of BBS4 antibody (82961-1-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-BBS4 transfected HEK-293 cells.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using BBS4 Recombinant antibody (82961-1-RR)

    IF Staining of hTERT-RPE1 using 82961-1-RR

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using BBS4 antibody (82961-1-RR, Clone: 230303C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

  • Flow cytometry (FC) experiment of HeLa cells using BBS4 Recombinant antibody (82961-1-RR)

    FC experiment of HeLa using 82961-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BBS4 Recombinant antibody (82961-1-RR, Clone:230303C2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82961-1-RR

    Affinity and Kinetic Characterization of 82961-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82961-1-RR against Human BBS4 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

BBS4 Recombinant antibody

BBS4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 82961-1-RR
Clone No.230303C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Bardet-Biedl syndrome 4 protein, Bardet Biedl syndrome 4, 230303C2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, SH-SY5Y cells
Positive IF/ICC detected inhTERT-RPE1 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82961-1-RR targets BBS4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BBS4 fusion protein Ag3430 相同性解析による交差性が予測される生物種
Full Name Bardet-Biedl syndrome 4
Calculated molecular weight 519 aa, 58 kDa
Observed molecular weight60 kDa
GenBank accession numberBC027624
Gene Symbol BBS4
Gene ID (NCBI) 585
RRIDAB_3670707
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96RK4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).

Protocols

Product Specific Protocols
WB protocol for BBS4 antibody 82961-1-RRDownload protocol
IF protocol for BBS4 antibody 82961-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols