Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | hTERT-RPE1 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-82961 targets BBS4 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | BBS4 fusion protein Ag3430 相同性解析による交差性が予測される生物種 |
| Full Name | Bardet-Biedl syndrome 4 |
| Calculated molecular weight | 519 aa, 58 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC027624 |
| Gene Symbol | BBS4 |
| Gene ID (NCBI) | 585 |
| RRID | AB_3673154 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96RK4 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 BBS4 antibody CL488-82961 | Download protocol |
| FC protocol for CL Plus 488 BBS4 antibody CL488-82961 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |