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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

    WB analysis using 16815-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

    WB analysis of A431 using 16815-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of RAW 264.7 cells using p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

    IP experiment of RAW 264.7 using 16815-1-AP

    IP result of anti-p130Cas / BCAR1 (IP:16815-1-AP, 4ug; Detection:16815-1-AP 1:600) with RAW 264.7 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

    IHC staining of human colon cancer using 16815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

    IHC staining of human colon cancer using 16815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

    IF Staining of HeLa using 16815-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using p130Cas / BCAR1 antibody (16815-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of A431 cells using p130Cas / BCAR1 Polyclonal antibody (16815-1-AP)

    FC experiment of A431 using 16815-1-AP

    1x10^6 A431 cells were intracellularly stained with 0.25 ug p130Cas / BCAR1 Polyclonal antibody (16815-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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p130Cas / BCAR1 Polyclonal antibody

p130Cas / BCAR1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 16815-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

BCAR1, CRK associated substrate, CASS1, CAS1, CAS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A431 cells, HepG2 cells, T-47D cells
Positive IP detected inRAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16815-1-AP targets p130Cas / BCAR1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p130Cas / BCAR1 fusion protein Ag10354 相同性解析による交差性が予測される生物種
Full Name breast cancer anti-estrogen resistance 1
Calculated molecular weight 870 aa, 93 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC062556
Gene Symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_2065460
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IHC protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IF protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IP protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
FC protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols