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Target

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Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated p130Cas / BCAR1 Polyclonal antibody

p130Cas / BCAR1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-16815

Synonyms

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated p130Cas / BCAR1 Polyclonal (CL555-16815)

    IF Staining of HeLa using CL555-16815

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 p130Cas / BCAR1 antibody (CL555-16815) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite®555-conjugated p130Cas / BCAR1 Polyclonal (CL555-16815)

    FC experiment of A431 using CL555-16815

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human p130Cas / BCAR1 (CL555-16815) (red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-16815 targets p130Cas / BCAR1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p130Cas / BCAR1 fusion protein Ag10354 相同性解析による交差性が予測される生物種
Full Name breast cancer anti-estrogen resistance 1
Calculated molecular weight 870 aa, 93 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC062556
Gene symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_2919632
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL555 p130Cas / BCAR1 antibody CL555-16815Download protocol
FC protocol for CL555 p130Cas / BCAR1 antibody CL555-16815Download protocol
Standard Protocols
Click here to view our Standard Protocols