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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated BCL2 Monoclonal antibody

BCL2 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig

Applications

IF/ICC, IF-P

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4H8C6

Cat no : CL555-60178

Synonyms

BCL-2, 4H8C6

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CoraLite®555-conjugated BCL2 Monoclonal antibody (CL555-60178)

    IF Staining of human appendicitis using CL555-60178

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CoraLite®555-conjugated BCL2 Monoclonal antibody (CL555-60178)

    IF Staining of human appendicitis using CL555-60178

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite®555-conjugated BCL2 Monoclonal antibody (CL555-60178)

    IF Staining of NIH/3T3 using CL555-60178

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®555-conjugated BCL2 Monoclonal antibody (CL555-60178)

    IF Staining of MCF-7 using CL555-60178

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman appendicitis tissue
Positive IF/ICC detected inNIH/3T3 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-60178 targets BCL2 in IF/ICC, IF-P applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BCL2 fusion protein Ag3508 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC027258
Gene symbol BCL2
Gene ID (NCBI) 596
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

1) What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.
2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.

Protocols

Product Specific Protocols
IF protocol for CL555 BCL2 antibody CL555-60178Download protocol
Standard Protocols
Click here to view our Standard Protocols